VVector Bio offers excellent services for Adeno-associated virus (AAV), Lentivirus and Adenovirus production to support cell and gene therapy development
Adenovirus vectors have multiple applications. It can be used in gene therapy, as vaccines, oncolytic virus, etc. At VVector Bio we have developed a manufacturing process rendering high yield of adenoviral vectors in HEK-293 suspension cells. The yields in crude cell culture supernatant are at 109 virus particles per mL.
Process package:
► Stock production
Single amplification round for adenoviral stock
► Production:
Infection of HEK-293 suspension cells at low MOI
Tailored fed-batch strategy and optimized process to obtain high yields
► Purification:
Concentration by cesium chloride ultracentrifugation
► Analytical:
Infection virus Particle concentration by TCID50
Total viral particles by anion-exchange HPLC method