VVector Bio

VVector Bio offers excellent services for Adeno-associated virus (AAV), Lentivirus and Adenovirus production to support cell and gene therapy development

Information

Adenovirus

  1. Home
  2. /
  3. Adenovirus

Adenovirus

 

 

Adenovirus vectors have multiple applications. It can be used in gene therapy, as vaccines, oncolytic virus, etc. At VVector Bio we have developed a manufacturing process rendering high yield of adenoviral vectors in HEK-293 suspension cells. The yields in crude cell culture supernatant are at 109 virus particles per mL.

 Process package:

► Stock production

  • Single amplification round for adenoviral stock

 ► Production:

  • Infection of HEK-293 suspension cells at low MOI
  • Tailored fed-batch strategy and optimized process to obtain high yields

 ► Purification:

  • Concentration by cesium chloride ultracentrifugation

► Analytical:

  • Infection virus Particle concentration by TCID50
  • Total viral particles by  anion-exchange HPLC method
  • ddPCR 
  • Total protein 
  • Host cell protein, Host cell DNA (on request)
  • Electronic microscopy (on request)


AAV Single Plasmids


Off-the-shelf AAV

Pre-clinical manufacturing

Process
Development

VVECTOR BIO, Bridging Research and Clinic

VVector Bio offers expert biomanufacturing services to accelerate the development and commercialization of novel medicines

Services

Useful Links

Information

2020 VVECTOR BIO. All Rights Reserved