VVector Bio offers excellent services for Adeno-associated virus (AAV), Lentivirus and Adenovirus production to support cell and gene therapy development
Our scientists have profound scientific knowledge and understanding of viruses and viral vaccines and have been involved in several successful vaccine development projects. We offer expert guidance to develop your project from design to final formulation.
Application Fields:
Vesicular Stomatitis Virus (VSV) and Newcastle Disease Virus (NDV) produced in Vero cells
Enveloped virus-like particles from HEK-293 cells and BV-insect cells
Influenza virus
Adenovirus (more information below)
Adenovirus
Adenovirus vectors have multiple applications. They can be used in gene therapy, as vaccines, oncolytic virus, etc.
At VVector Bio we have developed a manufacturing process rendering high yield of adenoviral vectors in HEK-293 suspension cells. The yields in crude cell culture supernatant are at 109 virus particles per mL.
Process package:
► Stock production
Single amplification round for adenoviral stock
► Production:
Infection of HEK-293 suspension cells at low MOI
Tailored fed-batch strategy and optimized process to obtain high yields
► Purification:
Concentration by cesium chloride ultracentrifugation
► Analytical:
Infection virus Particle concentration by TCID50
Total viral particles by anion-exchange HPLC method