Technology platforms

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Technology platforms

► HEK293 production platform

Most of our products are manufactured on a robust and high-performance HEK293 cell line adapted to chemically defined serum-free medium and suspension cultures, allowing batch-to-batch reproducibility, stability, safety and process control. On this platform, we are able to deliver products in high yields following the recommendation of regulatory agencies. Our HEK293 expression system can easily achieve high viable cell densities in a few days. Also, due to its easy infectability and transfectability, we are able to produce high titer viral vectors and stable HEK293 cell lines expressing your gene of interest for large-scale manufacturing. If your process requires a biomolecule with human-like post-translational modifications (glycosylation, hydroxylation, phosphorylation, proteolysis, etc) this is your system of choice as it presents reduced immunogenicity due to the absence of non-human α-galactose and N-glycolylneuraminic acid glycans.

On our HEK293 expression platform, we offer:

 

  •       Lentivirus
  •       Adenovirus
  •       Adeno-associated virus
  •       Recombinant Protein production
  •       Virus-like particles
  •       Influenza virus production

 

► CHO production platform                      

 

CHO cell line is a mammalian recombinant protein expression system with safety track-record, regulatory-friendly aspects and it is commonly used for protein manufacturing in industry. This system has the capability of producing proteins with similar human post-translational modifications. Different serum-free media have been developed for this cell line, allowing high yield products through process development and optimization. Similarly to HEK293, CHO cells have been adapted to suspension growth in chemically defined serum-free medium reaching high cell densities. CHO cells have high tolerance to biomanufacturing parameter changes such as pH, temperature, oxygen level, among others, which enable a robust recombinant protein production at high levels.
 On our CHO production platform, we offer:

 

  •       Recombinant Protein production

 

► SF9 insect cells-Baculovirus system

The dual Sf9 cell line-Baculovirus system is a fast, safe and versatile eukaryotic expression system commonly used to express recombinant proteins, virus-like particles  and AAVs at high levels. The main advantage of this system is its versatility in producing different baculoviruses carrying your gene of interest, which easily infect insect cells growing at high viable cell densities in bioreactors. These baculovirus vectors are produced by site-specific transposition of a foreign gene from a donor plasmid to a cloned baculoviral DNA called ‘bacmid’. Despite having an insect origin, Sf9 cells provide multiple post-translational modifications similar or identical to human counterparts. Also, Sf9 cells tolerate well culture parameter changes as osmolality and by-product concentration, making it a robust choice for your process. Our Sf9-BV system is adapted to grow in suspension culture using chemically defined serum-free medium . The gene of interest is regulated  by a strong insect promoter which drives high titers of the product.

On our Sf9-BV production platform, we offer:

 

  •       Adeno-associated virus
  •       Virus-like particles

 

Contact us for more information

VVECTOR BIO, Bridging Research and Clinic