Recombinant Protein Production

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Recombinant Protein Production

VVector Bio has experience in the production of recombinant proteins from HEK-293 suspension cells and CHO cells. The yields for recombinant proteins vary greatly depending on the protein of interest.

Process package:

 

► Plasmid Optimization

  • Promoters
  • Secretion signals, and 
  • Tags to facilitate the purification

► Production:

  • Transient transfection
  • Expression from a stable cell line (cell line development services offered)
  • Bioreactor production 1-10L (Fed-batch, perfusion)

► Purification:

  • TFF for concentration and diafiltration
  • Affinity and ion exchange chromatography 

► Analytical:

  • Total protein concentration, 
  • Purity and identity by (SDS-PAGE staining and WB) 
  • Recombinant protein concentration by ELISA, if available  
  • Endotoxin level (on request)
  • Host cell protein, Host cell DNA (on request).

VVECTOR BIO, Bridging Research and Clinic