VVector Bio has extensive experience in the manufacturing of AAV by triple transient transfection in HEK-293 suspension cells.
We have the plasmids for Rep/Cap for different serotypes including Rep2/Cap 2, 5, 6, 8, and 9 and the plasmid containing the adenovirus helper function. You can start your project with just the gene of interest (GOI) sequence in hand and we will provide you with the final genetic constructions, develop the production process, purification and perform the characterization and analytical quantification of the final product.
Furthermore, we have experience in house with cell assays to assess the functionality of the viral vectors using internal standards, so we can develop and validate your cell-based assay to determine the functional particles of your specific product.
VVector Bio also offers in-house technology for high-yield AAV production using the Baculovirus Insect cell system. We provide the molecular construction of the Bacmid containing the GOI and amplification of baculovirus stocks Bac-Rep, Bac-Cap and Bac-GOI followed by co-infection in SF9 cells, purification and characterization of the final product.